The Conversational Flow - An Official Podcast of KCAS

EXPLORE

Society & Culture

© 2024 PodJoint

https://is1-ssl.mzstatic.com/image/thumb/Podcasts211/v4/7f/3c/3a/7f3c3ab1-42a0-6052-c597-98733c2098c5/mza_1383707169721286208.jpg/600x600bb.jpg

The Conversational Flow - An Official Podcast of KCAS

KCAS

25 episodes

6 days ago

In episode 25 of "The Conversational Flow" podcast, guest David Leclerc, Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago, joins Dom and John to share his professional journey and insights into the evolving world of flow cytometry. Leclerc reflects on his early struggles during his master’s program in Montreal and how those challenges ultimately led him to a fulfilling career in core facility management and leadership. The discussion transitions to...

Show more...

All content for The Conversational Flow - An Official Podcast of KCAS is the property of KCAS and is served directly from their servers with no modification, redirects, or rehosting. The podcast is not affiliated with or endorsed by Podjoint in any way.

In episode 25 of "The Conversational Flow" podcast, guest David Leclerc, Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago, joins Dom and John to share his professional journey and insights into the evolving world of flow cytometry. Leclerc reflects on his early struggles during his master’s program in Montreal and how those challenges ultimately led him to a fulfilling career in core facility management and leadership. The discussion transitions to...

Show more...

Episodes (20/25)

The Conversational Flow - An Official Podcast of KCAS

What Happens if You Go with the Flow Too Much

In episode 25 of "The Conversational Flow" podcast, guest David Leclerc, Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago, joins Dom and John to share his professional journey and insights into the evolving world of flow cytometry. Leclerc reflects on his early struggles during his master’s program in Montreal and how those challenges ultimately led him to a fulfilling career in core facility management and leadership. The discussion transitions to...

3 weeks ago

1 hour 7 minutes

The Conversational Flow - An Official Podcast of KCAS

Considerations for Flow Cytometry-based Cell Sorting

In this episode, Adam and Brian explored recent advances in cell sorting technology, focusing on image-activated approaches. Unlike traditional flow cytometry, which relies on voltage signals, these systems capture and analyze cell images in real time to guide sorting decisions, enabling new insights such as subcellular localization and cell-to-cell signaling. The hosts emphasized trade-offs between purity, yield, and speed, as well as technical considerations like nozzle size, sample stabili...

1 month ago

57 minutes

The Conversational Flow - An Official Podcast of KCAS

Flow Cytometry: Not Just for Peripheral Blood Anymore

In episode #23 of The Conversational Flow, Adam and Brian tackle the question: When can flow cytometry be applied to non-blood matrices, and what must be considered? Using sputum analysis as a case study, they outline the challenges of clinical trial sample collection, emphasizing the importance of pre-analytical factors like consistency, bias, logistics, and handling. They discuss how panel design, reagent selection, and defining the right cell populations are critical starting points, follo...

2 months ago

47 minutes

The Conversational Flow - An Official Podcast of KCAS

The Nitty Gritty of Global Harmonization (w/ Special Guest, Karla Moncada)

In this episode, the hosts introduce Karla Moncada, who shares her professional journey from Flowcore management to her current role at KCAS, and unveils her new KCAS video series, Karla’s Corner, aimed at fostering year-round conversations within the flow cytometry community. Karla emphasizes the value of personal connections in scientific collaboration and how her career has blended customer service with cutting-edge science. The main topic of the episode focuses on global harmonization in ...

3 months ago

50 minutes

The Conversational Flow - An Official Podcast of KCAS

Flow Cytometry & Autoimmunity – A High Affinity Pharmacodynamic Match

In episode 21 of “The Conversational Flow” podcast, our hosts, Adam and Brian, explore the transformative power of gene therapy, starting things off by highlighting a compelling case involving CRISPR with milestones that once seemed impossible. They dive into the science behind CRISPR’s precise gene-editing capabilities and its potential for rapid, customizable treatments targeting rare and complex genetic mutations. The conversation also touches on the broader landscape of gene therapy deliv...

4 months ago

51 minutes

The Conversational Flow - An Official Podcast of KCAS

Impactful Developments Across Oncology and Regulatory Science

In this milestone 20th episode of The Conversational Flow, hosts Adam and Brian dive into impactful developments across oncology, regulatory science, and bioanalytical innovation. The episode highlig4 hts a major Keytruda study showing the first significant improvement in head and neck squamous cell carcinoma outcomes in over two...

5 months ago

53 minutes

The Conversational Flow - An Official Podcast of KCAS

Building on Backbone Panels: Standardization, Speed, and Integration

In the 19th episode of “The Conversational Flow”, our hosts, Brian and Adam, dive into the utility and innovation behind backbone panels in spectral flow cytometry, spotlighting a recent paper from BMS that presents a robust, flexible assay designed for immune monitoring using the Aurora platform. They discuss how backbone panels streamline assay development, enhance reproducibility, and are well-suited for integration with AI-driven gating strategies. The conversation also touches on industr...

6 months ago

1 hour 3 minutes

The Conversational Flow - An Official Podcast of KCAS

Flow Cytometry & Cell Therapy: One Cell Can Make a World of Difference

In this engaging episode of the podcast, hosts Brian Wile and Adam Cotty welcome special guests Michael Naso, Hillary Quinn, and Barry Morse from The Therapeutic Innovations Group—a powerhouse team of scientists and biotech veterans with decades of experience across biologics, small molecules, and cell therapy. The conversation dives deep into the guests’ unique career journeys, and their own consulting venture aimed at helping startups and academic teams navigate early-stage biotech developm...

7 months ago

1 hour 8 minutes

The Conversational Flow - An Official Podcast of KCAS

Flow Validations and Guidance Document Changes in 2024-2025

Episode 17 of The Conversational Flow podcast, hosted by Brian Wile and Adam Cotty, dives into the evolving landscape of flow cytometry networking and regulatory guidance, where they analyze recent FDA biomarker validation guidance, comparing it to existing industry standards like CLSI H62. Our hosts explore the challenges of harmonizing flow cytometry reporting, the role of regulatory clarity, and the potential for standardized data formats to drive the field forward. Packed with insights on...

8 months ago

45 minutes

The Conversational Flow - An Official Podcast of KCAS

The Role of Flow in Drug Autoimmune Indication Research

In episode 16 of The Conversational Flow podcast, hosts Brian Wile and Adam Cotty are joined by Allie Greenplate and Ayman Rezk to explore “The Role of Flow in Drug Autoimmune Indication Research.” The discussion delves into the surge in pharmaceutical R&D for immune and autoimmune indications, highlighting the influence of oncology treatments in shaping this trend. The guests shed light on how advancements in high-dimensional flow cytometry, particularly with more than 20-color capabilit...

9 months ago

1 hour

The Conversational Flow - An Official Podcast of KCAS

Fantastic Fluorophores and Where to Find Them (w/ Kelly Lundsten)

In this exciting 15th episode of The Conversational Flow podcast, titled "Fantastic Fluorophores and Where to Find Them," hosts Brian Wile and Adam Cotty from KCAS Bio are joined by special guest Kelly Lundsten from FluoroFinder. Together, they dive into the fascinating world of spectral flow cytometry, exploring key questions such as whether spectra or brightness is more critical for controls, which traditional fluorophores the field should consider moving away from, and what potential pitfa...

10 months ago

1 hour 5 minutes

The Conversational Flow - An Official Podcast of KCAS

Modifying Flow Cytometry Panels: the “Backbone” of Flow Cytometry

A flow cytometry backbone panel is a set of antibodies that can be used as a starting point for identifying cell types, and then supplemented with additional markers. On the most recent episode of “The Conversational Flow”, our hosts, Brian and Adam, spend some time together as they answer the questions of “What is a backbone panel?”, “What type of changes should be considered for a backbone panel?”, “What are the advantages to backbone panels for translational teams?”, “What should one be ca...

11 months ago

50 minutes

The Conversational Flow - An Official Podcast of KCAS

Low Content Analysis: When a smaller panel is better than a larger one

During the most recent episode of “The Conversational Flow” podcast, our hosts Brian and Adam talk about those times when a smaller panel might be desirable over a larger panel. To dive deep into this discussion and really analyze all the angles of these questions, the guys have invited a special guest to join them – Amrutesh Puranik, PhD. Amrutesh is the founder of Bespoke MultiOmics Consultants, and his perspective is very insightful as they answer the questions of “What are the benefits of...

1 year ago

1 hour 4 minutes

The Conversational Flow - An Official Podcast of KCAS

High Content Analysis: Finding Needles in a Growing Haystack

In the 12th episode of “The Conversational Flow” podcast, Brian and Adam welcome two special guests – Sean Burke of Denovo Software and Ryan Brinkman of Dotmatics – who join them to discuss the answers to a number of specific questions about High Content Analysis: How can we manage and derive insight from higher complexity panels? How can this be standardized? Once standardized, how can the process be automated? Why is more data challenging to deal with? How can we generate meaningful answers...

1 year ago

1 hour 3 minutes

The Conversational Flow - An Official Podcast of KCAS

Going Global with Spectral Flow Cytometry - US, EU and Now Australia

During this special episode of "The Conversational Flow" our hosts, Brian and Adam, welcome two guests: John Bucksath, CEO of KCAS Bio, and Stefan Cross, CEO of Crux Laboratories. These two guests discuss the elements and advantages of their organizations' new partnership between KCAS' US and European locations and Crux's location in Melbourne, Australia. Recent months have seen the three locations (Crux in Melbourne, and KCAS's Philadelphia and Lyon facilities) collaborate with Cytek to glob...

1 year ago

1 hour 6 minutes

The Conversational Flow - An Official Podcast of KCAS

Instrument Comparison: Some Instruments are More Equal than Others

When it comes to flow cytometry instrumentation, there are a number of things that can be different. You may have different types of lasers, you might have a different path for the laser to follow to get to the detector, you can have different actual detectors, different filters in front of those detectors, not to mention the varying sensitivities or levels of power for any of these items. In episode #10 of "The Conversational Flow", Brian and Adam discuss all of these while comparing them an...

1 year ago

55 minutes

The Conversational Flow - An Official Podcast of KCAS

Batch Effects, Controls, and Considerations for Longitudinal Studies

Let’s face it - nobody likes batch effects (unless it has to do with cookies, but that’s for another podcast). In episode #9 of “The Conversational Flow”, Brian and Adam talk about Batch Effects which is when you're having changes in the study that are not necessarily related to the scientific variables of the study – and what that might mean you will need to do next. Batch Effects can cause a number of headaches for anyone without experience with them… luckily both of our hosts have a number...

1 year ago

1 hour 4 minutes

The Conversational Flow - An Official Podcast of KCAS

Receptor Occupancy Testing in Clinical Trials

For episode #8 of “The Conversational Flow” podcast, Brian and Adam spend the time discussing Receptor Occupancy Testing in Clinical Trials. Receptor occupancy (RO) assays are important in clinical trials because they provide valuable information about a drug’s binding characteristics and mechanism of action. Our hosts begin by outlining the general need for accurate RO calculation (per time point, per subject, etc), then they move on to pre-analytical risks (collection, shipment, transit, re...

1 year ago

45 minutes

The Conversational Flow - An Official Podcast of KCAS

The Current State of Receptor Occupancy Validation

For episode #7 of “The Conversational Flow”, Brian and Adam invite David Ambrose to join them as they continue their conversation about Receptor Occupancy assays. There are so many unique considerations around an RO assay and with any flow cytometry assay, the precision of that assay is important. Our hosts discuss the biggest differentiators when you're starting to think about an RO assay and how to optimize the development of those assays and why it is so incredibly important to have a full...

1 year ago

1 hour

The Conversational Flow - An Official Podcast of KCAS

Receptor Occupancy: Deceptively Simple Questions

The newest episode of “The Conversational Flow” has Brian and Adam talking about the concepts around Receptor Occupancy and all the deceptively simple questions people often have about them, as well as the important but sometimes difficult answers to those questions. They talk about the biology of receptors and the few different "classes" associated with ROs. At their core, these are complex molecules with complex functions being discussed... and who better to have these complex discussions t...

1 year ago

38 minutes