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Howards Blend
Howard Blend
146 episodes
5 days ago
Each episode we explore topics that encourage free thought, promote wellness and taking responsibility for one's life.
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Self-Improvement
Education
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All content for Howards Blend is the property of Howard Blend and is served directly from their servers with no modification, redirects, or rehosting. The podcast is not affiliated with or endorsed by Podjoint in any way.
Each episode we explore topics that encourage free thought, promote wellness and taking responsibility for one's life.
Show more...
Self-Improvement
Education
Episodes (20/146)
Howards Blend
To Methylate Or Not To Methylate?

Discuss my thoughts on whether Dimethylglycine or Trimethylglycine is "better" for Homocysteine conversion to Methionine.This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice.Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.Resources: https://pmc.ncbi.nlm.nih.gov/articles/PMC4684116/https://cen.acs.org/articles/82/i3/METHYLMERCURY-TOXICOLOGY-PROBED.html#:~:text=%22Glutathione%20can%20be%20pumped%20out,brain%20as%20methylmercury%2Dglutathione.%22https://pmc.ncbi.nlm.nih.gov/articles/PMC4540061/https://www.sciencedirect.com/science/article/pii/S0163725821001182https://www.sciencedirect.com/science/article/pii/S0163725821001182 Image Resource:https://pmc.ncbi.nlm.nih.gov/articles/PMC6635095/ https://www.researchgate.net/figure/Overview-of-de-novo-glutathione-synthesis-Reduced-glutathione-or-GSH-is-a-tripeptide_fig2_5690064 https://lpi.oregonstate.edu/book/export/html/71 https://www.nature.com/articles/hr2011133 https://en.wikipedia.org/wiki/Serine_hydroxymethyltransferase - https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/methylenetetrahydrofolate-reductasehttps://chemtymology.co.uk/2020/11/27/cysteine-and-cystine/ https://fda.report/DailyMed/972f09a7-2aa1-4b1b-a6dc-2c4578a07803 https://www.thoughtco.com/definition-of-precipitate-604612 https://pubchem.ncbi.nlm.nih.gov/compound/Acetyl-p-benzoquinone-imine#section=3D-Conformer https://www.semanticscholar.org/paper/DRUG-METABOLISM-IN-THE-LIVER-Nguyen/4a29c6c4d433685a94b94602c012b8731cba5493https://pubchem.ncbi.nlm.nih.gov/compound/S-_Methylmercury_-L-Cysteine#section=2D-Structurehttps://keymerhealth.com/articles/seafood-mercury/https://onlinelibrary.wiley.com/doi/10.1155/2021/6564585 https://www.sciencedirect.com/science/article/abs/pii/S0304416519300133https://www.jbc.org/cms/10.1074/jbc.M111.265348/asset/822df563-eda2-4f48-a333-d01370a79e9c/main.assets/gr5_lrg.jpghttps://www.researchgate.net/figure/Betaine-metabolism-Metabolic-pathways-involving-betaine-BHMT-betaine-homocysteine_fig11_225081065https://www.acs.org/molecule-of-the-week/archive/c/l-cysteine.htmlhttps://biologydictionary.net/glutamic-acid/https://www.researchgate.net/figure/An-overview-of-the-reaction-catalyzed-by-SHMT-and-its-context-in-the-folate-cycle-A-The_fig1_338995871https://pubchem.ncbi.nlm.nih.gov/compound/5-Methyltetrahydrofolate#section=2D-Structurehttps://pubchem.ncbi.nlm.nih.gov/compound/Tetrahydrofolic-acid#section=2D-Structurehttps://pubchem.ncbi.nlm.nih.gov/compound/135398652#section=2D-Structure

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7 months ago
7 minutes 46 seconds

Howards Blend
A Vital Antioxidant

This episode goes over the compounds of N-acetylcysteine (NAC) and Glycine, and how they are used to make Glutathione. Also, we go over a couple of use cases for Glutathione. This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice.Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.Resources: https://pmc.ncbi.nlm.nih.gov/articles/PMC4684116/https://cen.acs.org/articles/82/i3/METHYLMERCURY-TOXICOLOGY-PROBED.html#:~:text=%22Glutathione%20can%20be%20pumped%20out,brain%20as%20methylmercury%2Dglutathione.%22https://pmc.ncbi.nlm.nih.gov/articles/PMC4540061/https://www.sciencedirect.com/science/article/pii/S0163725821001182https://www.sciencedirect.com/science/article/pii/S0163725821001182 Image Resource:https://pmc.ncbi.nlm.nih.gov/articles/PMC6635095/ https://www.researchgate.net/figure/Overview-of-de-novo-glutathione-synthesis-Reduced-glutathione-or-GSH-is-a-tripeptide_fig2_5690064 https://lpi.oregonstate.edu/book/export/html/71 https://www.nature.com/articles/hr2011133 https://en.wikipedia.org/wiki/Serine_hydroxymethyltransferase - https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/methylenetetrahydrofolate-reductasehttps://chemtymology.co.uk/2020/11/27/cysteine-and-cystine/ https://fda.report/DailyMed/972f09a7-2aa1-4b1b-a6dc-2c4578a07803 https://www.thoughtco.com/definition-of-precipitate-604612 https://pubchem.ncbi.nlm.nih.gov/compound/Acetyl-p-benzoquinone-imine#section=3D-Conformer https://www.semanticscholar.org/paper/DRUG-METABOLISM-IN-THE-LIVER-Nguyen/4a29c6c4d433685a94b94602c012b8731cba5493https://pubchem.ncbi.nlm.nih.gov/compound/S-_Methylmercury_-L-Cysteine#section=2D-Structurehttps://keymerhealth.com/articles/seafood-mercury/https://onlinelibrary.wiley.com/doi/10.1155/2021/6564585 https://www.sciencedirect.com/science/article/abs/pii/S0304416519300133

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7 months ago
7 minutes 55 seconds

Howards Blend
It’s Located Where?

Discussing a question I received asking where the blood brain barrier is located. In addition, we go over information about the blood csf barrier. This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice.Here's a link to the previous video on the blood brain barrier.https://spotifycreators-web.app.link/e/BQceRCwlKRbPlease like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.Resources:https://www.sciencedirect.com/topics/neuroscience/area-postrema#:~:text=The%20area%20postrema%20refers%20to,actions%20that%20lead%20to%20illness.https://qbi.uq.edu.au/brain/brain-anatomy/what-blood-brain-barrier#:~:text=The%20brain%20is%20precious%2C%20and,of%20the%20blood%E2%80%93brain%20barrier.https://www.cancer.gov/publications/dictionaries/cancer-terms/def/choroid-plexushttps://journals.lww.com/glaucomajournal/fulltext/2013/06001/production_and_circulation_of_cerebrospinal_fluid.5.aspxhttps://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-020-00230-3https://my.clevelandclinic.org/health/articles/22266-meningeshttps://pmc.ncbi.nlm.nih.gov/articles/PMC2821375/https://www.frontiersin.org/journals/neuroanatomy/articles/10.3389/fnana.2021.665803/full#F1https://pmc.ncbi.nlm.nih.gov/articles/PMC1871727/https://pmc.ncbi.nlm.nih.gov/articles/PMC7768803/https://pmc.ncbi.nlm.nih.gov/articles/PMC4120694/Image resources:https://www.cancer.gov/publications/dictionaries/cancer-terms/def/choroid-plexushttps://med.libretexts.org/Bookshelves/Anatomy_and_Physiology/Anatomy_and_Physiology_%28Boundless%29/11%3A_Central_Nervous_System/11.3%3A_Protection_of_the_Brain/11.3C%3A_Ventricleshttps://www.hydroassoc.org/understanding-the-choroid-plexus-function-location-and-its-role-in-hydrocephalus/https://www.researchgate.net/figure/blood-cerebrospinal-fluid-CSF-barrier-The-choroid-plexus-has-fenestrated-capillaries_fig1_325373010https://neuroscientificallychallenged.com/glossary/fourth-ventriclehttps://www.researchgate.net/figure/Area-Postrema-is-Anatomical-Marker-for-the-Subregion-of-NTS-of-Primary-Hypothetical_fig1_259320952https://www.rit.edu/spotlights/blood-brain-barrierhttps://www.cell.com/heliyon/fulltext/S2405-8440%2824%2911593-9https://www.aginganddisease.org/EN/10.14336/AD.2022.0130-1http://epilepsygenetics.net/the-epilepsiome/slc2a1-this-is-what-you-need-to-know/https://www.mdpi.com/2072-6643/16/14/2363https://www.mdpi.com/2072-6643/11/11/2636https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2019.00282/fullhttps://teachmephysiology.com/immune-system/innate-immune-system/phagocytosis/https://oehha.ca.gov/chemicals/methylmercury-and-methylmercury-compoundshttps://www.mdpi.com/2073-4409/11/18/2823https://www.simplypsychology.org/brain-ventricles.htmlhttps://www.frontiersin.org/files/Articles/123479/fnins-09-00032-HTML/image_m/fnins-09-00032-g001.jpghttps://www.researchgate.net/figure/Cerebrospinal-fluid-flow-Cerebrospinal-fluid-is-mainly-produced-in-the-lateral_fig2_370857837https://www.physio-pedia.com/CSF_Cerebrospinal_Fluid

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7 months ago
17 minutes 40 seconds

Howards Blend
BUM Energy Review

Going 2 Deep (two active ingredients) in this review of BUM Energy Drink, root beer flavor. This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice. Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Image References: https://www.mdpi.com/1420-3049/27/12/3679 https://courses.lumenlearning.com/wm-biology2/chapter/neural-stimulation-of-muscle-contraction/ https://link.springer.com/referenceworkentry/10.1007/978-3-030-42634-7_52 https://www.researchgate.net/figure/Tripartite-synapse-Participation-of-the-astrocyte-in-the-regulation-of-synapse-activity_fig3_366464810 https://www.researchgate.net/profile/Francisco-Wandosell/publication/258282849/figure/fig1/AS:203001995239428@1425410855933/The-coordinated-action-of-glial-and-neuronal-purinergic-receptors-and-purines-in-the-CNS.png https://www.researchgate.net/figure/Schematic-drawing-of-ATP-and-adenosine-receptor-localization-on-different-cell-types-in_fig2_284132757 https://www.mdpi.com/1422-0067/22/1/320 https://brainstuff.org/blog/cellular-action-of-adenosine-receptors https://www.osmosis.org/notes/Motor_Nervous_System#page-16 https://www.sciencefacts.net/resting-membrane-potential.html https://www.researchgate.net/figure/Sodium-Potassium-pump-which-uses-ATP-to-pump-sodium-ions-out-of-the-cell-The-hydrolysis_fig2_273751706 https://jackwestin.com/resources/mcat-content/nerve-cell/nodes-of-ranvier-propagation-of-nerve-impulse-along-axon https://old-ib.bioninja.com.au/standard-level/topic-6-human-physiology/65-neurons-and-synapses/synaptic-transfer.html https://www.oist.jp/image/exocytosis-and-endocytosis-are-key-steps-vesicle-recycling-exocytosis-increases-surface-area https://www.sciencelearn.org.nz/images/2456-caffeine-and-adenosine-structure

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9 months ago
24 minutes 5 seconds

Howards Blend
Crossing The BBB

This episode goes over my two cents about a question I received. Does lecithin cross the blood brain barrier more effectively than choline? This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice. Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Information References: https://www.sciencedirect.com/science/article/abs/pii/S0197018615300802?via%3Dihub https://pmc.ncbi.nlm.nih.gov/articles/PMC1997116 https://lpi.oregonstate.edu/mic/other-nutrients/choline https://pubmed.ncbi.nlm.nih.gov/14938439/ https://pmc.ncbi.nlm.nih.gov/articles/PMC6835836/ https://ods.od.nih.gov/factsheets/Choline-HealthProfessional/ Image References: https://cordancemedical.com/the-problem/blood-brain-barrier/ https://www.researchgate.net/figure/Molecular-characteristics-of-tight-junction-TJ-protein-complexes-at-the-blood-brain_fig1_363511691 https://www.sciencedirect.com/science/article/abs/pii/S1878818120304357 https://www.ncbi.nlm.nih.gov/books/NBK114308/ https://jissn.biomedcentral.com/articles/10.1186/1550-2783-4-5 https://www.ncbi.nlm.nih.gov/books/NBK594255/ https://pubchem.ncbi.nlm.nih.gov/compound/Choline https://pubchem.ncbi.nlm.nih.gov/compound/L-Tartaric-acid https://www.mdpi.com/2072-6651/8/11/326 https://pmc.ncbi.nlm.nih.gov/articles/PMC8235064/ https://www.examine.com/supplements/alpha-gpc/ https://pmc.ncbi.nlm.nih.gov/articles/PMC10025538/ https://pressbooks.calstate.edu/nutritionandfitness/chapter/6-2-phospholipids-and-sterols/ https://lpi.oregonstate.edu/mic/other-nutrients/choline

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9 months ago
9 minutes 7 seconds

Howards Blend
Lecithin VS Choline Part 2

This is the second of a 2 part response to a question about lecithin vs choline. Here's the link to part 1. https://open.spotify.com/episode/2at2pXZQ02Wt9ZmYq3lczz?si=6exmen2-QjWM2fJ6aaItog Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Information References: https://www.sciencedirect.com/science/article/abs/pii/S0197018615300802?via%3Dihub https://pmc.ncbi.nlm.nih.gov/articles/PMC1997116 https://lpi.oregonstate.edu/mic/other-nutrients/choline https://pubmed.ncbi.nlm.nih.gov/14938439/ https://pmc.ncbi.nlm.nih.gov/articles/PMC6835836/ https://ods.od.nih.gov/factsheets/Choline-HealthProfessional/ Image References: https://cordancemedical.com/the-problem/blood-brain-barrier/ https://www.researchgate.net/figure/Molecular-characteristics-of-tight-junction-TJ-protein-complexes-at-the-blood-brain_fig1_363511691 https://www.sciencedirect.com/science/article/abs/pii/S1878818120304357 https://www.ncbi.nlm.nih.gov/books/NBK114308/ https://jissn.biomedcentral.com/articles/10.1186/1550-2783-4-5 https://www.ncbi.nlm.nih.gov/books/NBK594255/ https://pubchem.ncbi.nlm.nih.gov/compound/Choline https://pubchem.ncbi.nlm.nih.gov/compound/L-Tartaric-acid https://www.mdpi.com/2072-6651/8/11/326 https://pmc.ncbi.nlm.nih.gov/articles/PMC8235064/ https://www.examine.com/supplements/alpha-gpc/ https://pmc.ncbi.nlm.nih.gov/articles/PMC10025538/ https://pressbooks.calstate.edu/nutritionandfitness/chapter/6-2-phospholipids-and-sterols/ https://lpi.oregonstate.edu/mic/other-nutrients/choline https://www.researchgate.net/figure/molecular-structure-of-the-phosphatidylethanolamine-eluting-at-462-min_fig2_23771336 https://www.nature.com/articles/srep16787 https://www.researchgate.net/figure/Betaine-metabolism-Metabolic-pathways-involving-betaine-BHMT-betaine-homocysteine_fig11_225081065 https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2022.1109445/full https://ncbi.nlm.nih.gov/books/NBK554408/ https://www.researchgate.net/figure/A-simplified-illustration-of-the-homocysteine-metabolism-pathway-BHMT-betaine_fig1_341362341 https://www.researchgate.net/figure/Fig-2-The-nucleus-basalis-of-Meynert-innervates-the-entire-cerebral-mantle-with_fig1_327756974 https://www.researchgate.net/figure/The-human-cholinergic-system-Cholinergic-nuclei-are-primarily-located-in-numbered-cell_fig1_345346799

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9 months ago
9 minutes 45 seconds

Howards Blend
Lecithin VS Choline Part 1

This is the first of a two part response to a question about lecithin vs choline. Stay tuned for part 2. Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Information References: https://www.sciencedirect.com/science/article/abs/pii/S0197018615300802?via%3Dihub https://pmc.ncbi.nlm.nih.gov/articles/PMC1997116 https://lpi.oregonstate.edu/mic/other-nutrients/choline https://pubmed.ncbi.nlm.nih.gov/14938439/ https://pmc.ncbi.nlm.nih.gov/articles/PMC6835836/ https://ods.od.nih.gov/factsheets/Choline-HealthProfessional/ Image Resources: https://cordancemedical.com/the-problem/blood-brain-barrier/ https://www.researchgate.net/figure/Molecular-characteristics-of-tight-junction-TJ-protein-complexes-at-the-blood-brain_fig1_363511691 https://www.sciencedirect.com/science/article/abs/pii/S1878818120304357 https://www.ncbi.nlm.nih.gov/books/NBK114308/ https://jissn.biomedcentral.com/articles/10.1186/1550-2783-4-5 https://www.ncbi.nlm.nih.gov/books/NBK594255/ https://pubchem.ncbi.nlm.nih.gov/compound/Choline https://pubchem.ncbi.nlm.nih.gov/compound/L-Tartaric-acid https://www.mdpi.com/2072-6651/8/11/326 https://pmc.ncbi.nlm.nih.gov/articles/PMC8235064/ https://www.examine.com/supplements/alpha-gpc/ https://pmc.ncbi.nlm.nih.gov/articles/PMC10025538/ https://pressbooks.calstate.edu/nutritionandfitness/chapter/6-2-phospholipids-and-sterols/ https://lpi.oregonstate.edu/mic/other-nutrients/choline https://www.researchgate.net/figure/molecular-structure-of-the-phosphatidylethanolamine-eluting-at-462-min_fig2_23771336 https://www.nature.com/articles/srep16787 https://www.researchgate.net/figure/Betaine-metabolism-Metabolic-pathways-involving-betaine-BHMT-betaine-homocysteine_fig11_225081065 https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2022.1109445/full https://ncbi.nlm.nih.gov/books/NBK554408/ https://www.researchgate.net/figure/A-simplified-illustration-of-the-homocysteine-metabolism-pathway-BHMT-betaine_fig1_341362341 https://www.researchgate.net/figure/Fig-2-The-nucleus-basalis-of-Meynert-innervates-the-entire-cerebral-mantle-with_fig1_327756974 https://www.researchgate.net/figure/The-human-cholinergic-system-Cholinergic-nuclei-are-primarily-located-in-numbered-cell_fig1_345346799

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9 months ago
7 minutes 48 seconds

Howards Blend
Increased Absorption?

Replying to a question about how the absorption of choline be improved. This was a response on a choline bitartrate video, so I used that ingredient to explain. Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Image Resources: https://onlinelibrary.wiley.com/doi/10.1155/2021/6564585 https://socratic.org/questions/how-is-the-structure-of-a-villi-in-the-small-intestine-related-to-its-function

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10 months ago
3 minutes 48 seconds

Howards Blend
Antihistamines And Choline

Answering a question regarding antihistamines and the effects on acetylcholine receptors. Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible. Resources: https://www.jacionline.org/article/S0091-6749(06)02977-0/fulltext https://www.ncbi.nlm.nih.gov/books/NBK557790/ https://www.ncbi.nlm.nih.gov/books/NBK538188/ https://pubmed.ncbi.nlm.nih.gov/11164999/ https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.14696 https://www.ncbi.nlm.nih.gov/books/NBK526010/ Image Resources: https://www.ebiohippo.com/en/aav-actone-gpcr-assay-kits.html https://www.science.org/doi/10.1126/science.aaw5188 https://meducatmedical.com/what-is-continence/

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10 months ago
6 minutes 59 seconds

Howards Blend
Citicoline Day Or Night?

Discussing two questions I received. At what time should you take Citicoline? Also, does it work for the nervous system? Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.

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10 months ago
6 minutes 21 seconds

Howards Blend
Ghost Energy - Taste Test

Going over the taste of Ghost Energy Welch's Grape drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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11 months ago
2 minutes 10 seconds

Howards Blend
Ghost Energy Drink Review

Complete review of  Ghost Energy Welch's Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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11 months ago
23 minutes 32 seconds

Howards Blend
Ghost Energy - Vitamins

Discussing the vitamins in Ghost Lifestyle's Ghost Energy Welch's Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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1 year ago
5 minutes 26 seconds

Howards Blend
Ghost Energy Review - Astragin

This video covers the sixth ingredient of Ghost Lifestyle's Ghost Energy Welch’s Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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1 year ago
3 minutes 1 second

Howards Blend
Ghost Energy Review - Neurofactor

This video covers the fifth ingredient of Ghost Lifestyle's Ghost Energy Welch’s Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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1 year ago
3 minutes 12 seconds

Howards Blend
Ghost Energy Review - Alpha GPC

This video covers the fourth ingredient of Ghost Lifestyle's Ghost Energy Welch’s Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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1 year ago
2 minutes 23 seconds

Howards Blend
Ghost Energy Review - Natural Caffeine

This video covers the third ingredient of Ghost Lifestyle's Ghost Energy Welch’s Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review. #supplements #supplementreview

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1 year ago
3 minutes 21 seconds

Howards Blend
Ghost Energy Review - Taurine

This video covers the second ingredient of Ghost Energy Welch’s Grape flavored drink. Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.

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1 year ago
2 minutes 19 seconds

Howards Blend
Ghost Energy Review - L-Carnitine L-Tartrate

Welcome to the first supplement review of this channel.


This video covers the first ingredient of Ghost Energy Welch’s Grape flavored drink.


Please like, comment and share if you find value in this video. Also, let me know of any supplements you’d like me to review.


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1 year ago
1 minute 34 seconds

Howards Blend
Decreasing Choline Intake?

Replying to a comment I received asking how to decrease Choline intake. Disclaimer: This video is strictly for educational purposes. It is not a substitute for seeking professional medical help.
Have Questions/ Comments? I can answer them in an upcoming episode. Instagram: https://www.instagram.com/howardsblend/ TikTok https://www.tiktok.com/@howardsblend

Send me an email smbsupps@gmail.com Resources: https://ods.od.nih.gov/factsheets/Choline-HealthProfessional/#h3 https://lpi.oregonstate.edu/mic/dietary-factors/L-carnitine#food-sources https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302777/

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1 year ago
4 minutes 26 seconds

Howards Blend
Each episode we explore topics that encourage free thought, promote wellness and taking responsibility for one's life.