In this episode, we travel back to one of the great origin stories in gene regulation: the discovery of lin-4, the first-ever microRNA. In Caenorhabditis elegans, scientists found that tiny non-coding RNAs could silence gene expression by pairing with target mRNAs — launching the entire field of microRNA biology.

We explore:

• How lin-4 regulates developmental timing by repressing LIN-14 protein

• The discovery of small RNAs (22 and 61 nucleotides) as gene regulators

• The first evidence for RNA-RNA antisense interactions controlling translation

• Why this work reshaped our understanding of gene expression across species

• How a worm taught us that not all genes code for proteins

📖 Based on the research article:
"The C. elegans Heterochronic Gene lin-4 Encodes Small RNAs with Antisense Complementarity to lin-14"
Rosalind C. Lee, Rhonda L. Feinbaum & Victor Ambros.
Published in Cell (1993).
🔗 https://doi.org/10.1016/0092-8674(93)90529-Y

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