Abstract: The rapid diffusion of generative artificial intelligence tools is fundamentally reshaping professional boundaries within organizations. As accessible AI systems enable individuals to perform tasks previously requiring specialized training—coding, design, content creation, data analysis—organizations face a novel form of role conflict driven not by resource scarcity but by capability abundance. This article examines AI-driven role conflict as an emergent organizational phenomenon characterized by tension between traditional role boundaries and AI-enabled capability expansion. Drawing on research from organizational behavior, human-computer interaction, and change management, we analyze how this capability democratization creates both acceleration opportunities and defensive retrenchment. Evidence from multiple industries reveals that organizations respond along a spectrum from territorial protection to deliberate role fluidity experimentation. We propose evidence-based interventions including transparent reskilling pathways, contribution-based evaluation frameworks, and collaborative workflow redesign. Long-term organizational resilience requires psychological contract recalibration, distributed expertise models, and continuous learning systems that acknowledge AI as a capability amplifier rather than role replacement. Organizations that proactively address these tensions can harness cross-functional acceleration while preserving specialized expertise depth.