On this episode, Gema Chacón sheds some light on the diagnosis and interpretation of Leptospira spp. The diagnosis of this pathogen has commonly been done by the MAT, an indirect technique that only shows contact with the bacterium. In recent years, new direct diagnostic tools such as the qPCR have become available even the sequencing of the implied serovar in case of positive cases. Gemma speaks about when to use each method, consider the complicated epidemiology of this bacterium, and how to interpretate the different results obtained.