This research article examines the content of heavy metals and mycotoxins, as well as the bioaccessibility of minerals in extracts from rainbow trout and sole by-products obtained through pressurized liquid extraction (PLE). The study evaluates the effect of in vitro gastrointestinal digestion on antioxidant capacity and mineral bioaccessibility. The results show that contaminant levels are below legal limits, while PLE enhances the recovery of antioxidants and certain minerals. Finally, the bioaccessibility of minerals after simulated digestion is analyzed, revealing variable effects depending on the type of mineral and fish species. DOI: https://doi.org/10.1016/j.foodchem.2023.136054

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