In this episode, we delve into a study titled “Functions, Interactions, and Prognostic Role of POLE: A Bioinformatics Analysis” by Carvajal-Veloza and colleagues, which explores the impact of POLE mutations in endometrial cancer. Using advanced bioinformatics tools, the researchers analyzed 30 mutations in the exonuclease domain of the POLE gene, highlighting their pathogenic effects on protein structure, DNA replication, and repair mechanisms. The study also investigated clinical data from over 2,900 endometrial cancer cases, finding that POLE mutations are more frequent in microsatellite-unstable tumors and are associated with higher infiltration of immune cells such as CD8+ T cells and macrophages, indicating enhanced antitumoral immune activity. Despite contributing to genomic instability and potential platinum resistance, these mutations are linked to favorable prognoses due to their immune-activating effects. This discussion underscores the critical role of molecular profiling in improving treatment strategies for endometrial cancer.

Functions, Interactions and Prognostic role of POLE: a bioinformatics analysis (https://doi.org/10.3802/jgo.2025.36.e45)