This research article describes RemAIN, a prophage competition element in Salmonella that protects the bacteria from lysis by preventing the completion of lytic cycles in co-resident prophages. RemAIN achieves this through tRNA cleavage, specifically targeting tRNAs at the anticodon loop. The study shows RemAIN's activity is crucial in intracellular Salmonella persisters within macrophages, influencing both bacterial persistence and the host immune response. Furthermore, the authors identify a second prophage competition element, PRTase-DprA, in another Salmonella strain, suggesting that such elements may be common in this bacteria. The findings highlight a complex interplay between prophages, bacterial survival, and the host immune system.