This report from the RN2Blend consortium presents findings from a five-year study on differentiated nursing practices in Dutch hospitals. The research explores how hospitals are implementing varied roles for nurses, the challenges they encounter, and the effects on professional development, care quality, and cost-effectiveness. The study demonstrates that differentiated working leads to increased nurse involvement in policy and quality improvement, and can improve nurse retention, but requires the engagement of various stakeholders and ongoing investment. It highlights the necessity to consider historical contexts, power dynamics, and the diversity of nurses’ ambitions when implementing differentiated practices, emphasising that it is not a one-size-fits-all solution and that the effects depend on the context. The study concludes that while there have been positive steps, further support is needed to ensure the sustainable embedding of new roles, enhanced nurse empowerment, and improved team collaboration.