SPOT Synthesis and Peptide Arrays

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Exploration Science with Dr. Wendy Hartsock and Dr. Diedra Shorty

Dr. Wendy Hartsock and Dr. Diedra Shorty

45 episodes

3 days ago

Exploration Science is a discussion-based interview series in which scientists talk about their ventures and areas of expertise to educate and inspire other scientists. Join CEM's Dr. Wendy Hartsock and Dr. Diedra Shorty as they interview brilliant researchers to discuss peptides, proteins, and cutting-edge science.

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SPOT Synthesis and Peptide Arrays

Exploration Science with Dr. Wendy Hartsock and Dr. Diedra Shorty

48 minutes 6 seconds

2 years ago

SPOT Synthesis and Peptide Arrays

In this episode of Exploration Science, Drs. Ronald Frank (AIMS Scientific Products GmbH) and Christian Behn (CEM) discuss the evolution of SPOT Synthesis to generate peptide libraries. They also correct some misinformation and bring to light the ease and utility of applying SPOT arrays to biological studies.

Watch on YouTube: https://youtu.be/MBJFIygXDRg

Correction: The founding of AIMS company was in 1998, not 1989.

See the links and references below for some examples of SPOT applications:

AIMS Scientific Products http://www.aims-scientific-products.de/index.php

CEM MultiPep: https://cem.com/multipep2

Clemens Schulte, Vladimir Khayenko, Amit Jean Gupta, Hans Michael Maric. Low-cost synthesis of peptide libraries and their use for binding studies via temperature-related intensity change. STAR Protocols. Volume 2, Issue 3, 2021, 100605. https://doi.org/10.1016/j.xpro.2021.100605.

Melder FTI, Lindemann P, Welle A, Trouillet V, Heißler S, Nazaré M, Selbach M. Compound Interaction Screen on a Photoactivatable Cellulose Membrane (CISCM) Identifies Drug Targets. ChemMedChem. 2022 Oct 6;17(19):e202200346. doi: 10.1002/cmdc.202200346. Epub 2022 Sep 20. PMID: 35867055; PMCID: PMC9826412.

Mohammed El-Mowafy, Ursula Bilitewski. Identification of possible Ser/Thr/Tyr phosphorylation sites in the fungal histidine kinase CaNik1p by peptide array technique. Bulletin of Faculty of Pharmacy, Cairo University. Volume 56, Issue 1, 2018, Pages 68-72. https://doi.org/10.1016/j.bfopcu.2018.03.007.

Moreno-Yruela, C., Bæk, M., Vrsanova, AE. et al. Hydroxamic acid-modified peptide microarrays for profiling isozyme-selective interactions and inhibition of histone deacetylases. Nat Commun 12, 62 (2021). https://doi.org/10.1038/s41467-020-20250-9.

Meyer et al., Mutations in Disordered Regions Can Cause Disease by Creating Dileucine Motifs. Cell (2018), https://doi.org/10.1016/j.cell.2018.08.019.

Rebollo, A., Fliedel, L., Tuffery, P., PEPscan: A Broad-Spectrum Approach for the Characterization of Protein-Binder Interactions? Biomolecules 2022, 12, 178. https://doi.org/10.3390/

Rosie M. Jarrald, Aw W. Liang Alvin, Andrea E. Rawlings, Masayoshi Tanaka, Mina Okochi, and Sarah S. Staniland. Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides. Bioconjugate Chemistry 2020 31 (8), 1981-1994. DOI: 10.1021/acs.bioconjchem.0c00348.