Pushing spaghetti through holes
Chapers:
00:00 Intro
11:15 Measuring Ions
17:00 Quality
23:00 Duplex
24:30 MinION
25:15 PromethION
28:20 Methylation
29:00 Direct RNA sequencing
38:25 Basecalling
46:00 Basecalling bias
53:50 Sequencing proteins
- Nanopore explainer: https://nanoporetech.com/applications/dna-nanopore-sequencing
- Oxsci explaining the technology:https://oxsci.org/pore-over-this-advances-in-dna-sequencing/
- History of Nanopore:https://www.whatisbiotechnology.org/index.php/science/summary/nanopore/nanopore-sequencing-makes-it-possible-to-decode-the
- Shmou’s Biology explaining Nanopore sequencing:https://www.youtube.com/watch?v=MlluAjhzXqI
- A direct comparison of Nanopore and Illumina output:https://medresearch.umich.edu/office-research/about-office-research/biomedical-research-core-facilities/advanced-genomics-core/technologies/next-generation-sequencing
- A nice Nanopore QC tool we use: https://hpc.nih.gov/apps/pycoQC.html
- How a MinION looks like:https://www.whatech.com/og/markets-research/medical/658327-global-nanopore-sequencing-market-2020
Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany.
Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).
Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG(https://www.dfg.de/) (No. 407493903).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
