In this episode of BioSpeak, we examine the formation and fusion of vesicles, detailing the precise steps and protein interactions that enable cellular transport. We explain the endocytosis process, where proteins like clathrin, adaptin, and dynamin coordinate to form and release a vesicle from the cell membrane. Moving between the ER and Golgi apparatus, we outline the unique roles of COPI and COPII proteins in retrograde and anterograde transport. The episode concludes with an overview of vesicle docking and fusion mechanisms, focusing on the critical roles of Rab-GTPase, Rab effectors, and SNARE proteins in guiding vesicles to their target membranes.